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Scanning Electron Microscopy of Cerebellar Cortex by Orlando Castejon (Paperback, 2003)

thenilestore (1242003)

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Conventional SEM technique or slicing technique. - Fixation procedures. - The prefixation state of the nerve tissue. - Vascular perfusion fixation technique. - Trimming procedure and obtaining nerve tissue slices.

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In a clear exposition this book shows the in situ three-dimensional morphology of cerebellar neurons, intracortical circuits and synaptic connections that underpin the functioning of cerebellar neurons in networks. It brings together in one volume a new view of the three-dimensional cytoarchitectonic arrangement of the cerebellar cortex. The book shows the cerebellar extrinsic and intrinsic intracortical circuits formed by mossy and climbing fibres as exposed by the cryofracture methods. The high degree of lateral collateralization of these fibres is also displayed providing new insights on the information processing in the cerebellar cortex. Besides, field emission high resolution electron microscopy shows its potential contribution to the study of synaptic morphology. The concluding chapter deals with the contribution of scanning electron microscopy to cerebellar neurobiology. This monograph is an authoritative survey and a must for anyone who is interested in the structure of the central nervous system. It will also appeal to an interdisciplinary audience who wants to learn more about electron microscopy and neurocytology.